Generator

Part:BBa_K914013:Design

Designed by: Denis Samuylov   Group: iGEM12_Paris_Bettencourt   (2012-10-24)


RFP controlled by pRha


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 704
    Illegal AgeI site found at 816
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

pRha: one base pair modified (90: A -> T) to avoid EcoRI site. Mutation made in a less conserved base pair.


Source

Mutatated version of pRha was synthesized (gBlock).

References